Clustered DNA damage induced by gamma radiation in human fibroblasts (HF19), hamster (V79-4) cells and plasmid DNA is revealed as Fpg and Nth sensitive sites

Radiation - induced base damage – FPG - sensitive sites in plasmid DNA

Major progress of the Heidelberg ion beam cancer ther-apy centre took place in 2007. The first turn of the beamin the synchrotron was reached on February, 7th. Firstbeam in a treatment room was achieved on March, 20th.Until December, 16ththe beam performance in the firsttwo treatment rooms was pushed to the quality which isrequired for patient tre...

Radiation-induced base damage – FPG-sensitive sites in plasmid DNA

Radiation-induced damage in DNA depends on the pattern of deposited energy – homogeneous in case of X-rays and very inhomogeneous for charged particles (energy deposited along their tracks in a correlated way). It is accepted that the biological effect, its complexity and severity, induced by lowand high-LET radiation (X-rays and charged particles, respectively) is strongly dependent on the dis...

Radiation track and DNA damage

DNA probe in V79 Chinese hamster cells

Coordination complexes of type [Ru(L)3] , where L is a nitrogen-containing aromatic bidentate ligand, can often be photolytically reduced, making them useful in studies of DNAor protein-mediated electron transfer and in artifical photosynthesis model systems. Upon binding to DNA some Ru(L) complexes have been found to display strongly increased fluorescence compared with when free in solution, ...

DNA Damage in Leukocytes from Fanconi Anemia (FA) Patients and Heterozygotes Induced by Mitomycin C and Ionizing Radiation as Assessed by the Comet and Comet-FISH Assay

Background: Lymphocytes of Fanconi anemia (FA) show an increased sensitivity to the alkylating agents such as mitomycin C (MMC), but their responses to gamma-irradiation is controversial. The extent of DNA damage in leukocytes of FA patients following irradiation and MMC treatment was studied at cellular and single chromosome level. Methods: DNA damage induced by gamma-rays and MMC was measure...